Q Imaging Rolera Thunder User Manual

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Imaging Software
cellSens
Seamless Workflow. Intuitive Operation.
Compatible image formats
Read and write JPEG, JPEG2000, TIFF, BMP, AVI, PNG, VSI (Virtual slide image),
Read only GIF, PSD (Adobe Photoshop), TIFF (DP-BSW, FSX100, MetaMorph), OIF/OIB (Fluoview format), Cell, STK (MetaMorph), MRC (Medical Research Council)
• OLYMPUS CORPORATION is ISO14001 certified.
• OLYMPUS CORPORATION is FM553994/ISO9001 certified.
• All company and product names are registered trademarks and/or trademarks of their respective owners. • Images on the PC monitors are simulated.
• Specifications and appearances are subject to change without any notice or obligation on the part of the manufacturer.
48 Woerd Avenue Waltham, MA, 02453, USA.
Wendenstrasse 14-18, 20097 Hamburg, Germany
Shinjuku Monolith, 2-3-1, Nishi Shinjuku, Shinjuku-ku, Tokyo, 163-0914, Japan
491B River Vallay Road, #12-01/04 Valley Point Office Tower, Singapore 248373
3 Acacia Place, Notting Hill VIC 3168, Australia
5301 Blue Lagoon Drive, Suite 290 Miami, FL 33126, U.S.A.
A8F, Ping An International Financial Center, No. 1-3, Xinyuan South Road,
Chaoyang District, Beijing, 100027 P.R.C.
8F Olympus Tower, 446 Bongeunsa-ro, Gangnam-gu, Seoul, 135-509 Korea
Not for clinical diagnostic use.
Printed in Japan
M1718E-042014
Products with confirmed functionality
Dimension Standard Entry
Olympus
Camera DP20*
1
, DP21, DP25, DP26, DP70*
1
, DP71*
2
, DP72, DP73*
3
, DP80*
3
3 3 3
Micoscope
BX43, BX53, BX63, BX61, BX61WI, IX83, IX73, IX81, SZX16A
3 3
IX81-ZDC, IX81-ZDC2, IX3-ZDC
3
Peripherals BX-DSU, IX3-DSU, IX2-DSU, U-CBF
3
Motorized XY stage BX3-SSU, IX3-SSU Multiposition
Olympus Soft
Imaging Solutions
Camera
CC12, F-View II, Colorview I, Colorview II, Colorview III, Colorview IIIu,
XM10, XC10, XC30, XC50, UC30, UC50, SC20, SC30, SC100
3 3 3
Peripherals
cell^TIRF (multi-line, single line), MT20, USB-ODB converter,
Real Time Controller (U-RTC and U-RTCE), U-FCB
3
Hamamatsu Camera
Orca R2 (C10600-10B), Orca 03 (C8484-03G), Orca 05 (C8484-05G),
Orca ER (C4742-95-12ER), Orca Flash 2.8
3
ImagEM C9100-13, ImagEMX2(C9100-23B), ORCA-Flash 4.0 V2(C11440-22CU)
High-End Camera
Q-Imaging Camera
MicroPublisher 3.3 RTV, MicroPublisher 5 RTV
3 3
Monochrome: Exi Blue/Aqua, RETIGA (Exi, SRV, 2000R, 2000RV, 4000R,
4000RV, 6000_mono) QIClick plus RGB slider
3
Color : Exi Aqua
Rolera Thunder
High-End Camera
Photometrics
Camera
CoolSNAP HQ2
3
Evolve 512 Delta
High-End Camera
Image Splitter Dual View DV2 /QuadView QV2
Ratio/High End Device
Andor Camera iXon X3 897, iXon Ultra 897, Zyla4.2
High-End Camera
Jenoptik Camera ProgRes C3, ProgRes C5
3 3
Vincent Associates Shutter Uniblitz shutter (VCM-D1, VMM-D1, VMM-D3)
3 3
CoolLED Light Source precisExcite (pE-1, pE-2)
3
Lumen Dynamics Light Source X-Cite 120 PC, X-Cite exacte, X-Cite XLED1
3
Sutter
Light Source Lambda DG4
3
Shutter, FW Lambda 10-3/10-B
3
Prior
Motorized XY stage Proscan (I, II, III), Optiscan Multiposition
Shutter, FW, Z-drive Proscan (I, II, III), Optiscan II
3
Piezo Z (control via Real Time Controller)
NanoScanZ NZ100 High-End Device
Ludl
Motorized XY stage Mac 6000 Multiposition
Shutter, FW, Z-drive Mac 6000
3
Objective Imaging
Motorized XY stage controller Oasis 4i Multiposition
Z-drive controller Oasis 4i
3
Märzhäuser
Motorized XY stage Tango Multiposition
Z-drive controller Tango
3
Physik Instrumente
Piezo Z (control via Real Time Controller)
PIFOC P-721
High-End Device
Yokogawa CSU CSU-X1 High-End Device
*
1
DP20/70 does not support Windows7 64bit, Windows 8 32bit/64bit. *
2
DP71 does not support Windows8 32bit. *
3
DP73/80 support only Windows7/8 64bit.
Recommended system requirements
OS Microsoft Windows 8 (32-bit/64-bit) Pro, Microsoft Windows 7 (32-bit/64-bit) Ultimate with SP1, Microsoft Windows 7 (32-bit/64-bit) Professional with SP1
OS Language English, Simplified Chinese, Japanese, German, Russian (only for Entry and Standard) and all others with English like alphabet
CPU Intel Core i5, Intel Core i7, Intel Xeon
Recommended for high speed image acquisition: QuadCore
RAM 4 GB Recommended for high speed image acquisition: 8GB or more only on Windows7 64-bit operating system
Graphic card 1280x1024 (min. 1024 x768) monitor resolution with 32-bit-video card with separate graphics memory (no integrated graphics processor with shared memory)
Port
USB 2.0 port to connect devices to the system Fire Wire A to connect devices to the system (BX61, IX81, SZX2-MDCU, IX3-DSU etc...) Serial (RS232) to connect devices to the system (BX61,
IX81, SZX2-MDCU etc...) Additional PCI/PCIe slots as necessary to connect third party peripherals (principally third party cameras) with proprietary interface cards
HDD 1 GB for installation Performance of hard disk is a limiting factor for image acquisition speed Recommended for high speed image acquisition: Solid State Drive (SSD)
Drive DVD drive (Read: DVD-R DL)
Web Browser Recommended for Windows 7: Microsoft Internet Explorer 8.0, 9.0, Recommended for Windows 8: Microsoft Internet Explorer 10
cellSens functions
Dimension Standard Entry
Layout User experience customization
3 3 3
View
Overlay multiple images
3 3
Document groups for side-by-side image comparison
3 3 3
Movie playback
3 3 3
Tile view (multiple images in a single data set shown side by side)
3 3 3
Slice view for orthogonal plane viewing of 3D or time-lapse data sets
3
Voxel view for isosurface and volumetric rendering of 3D and 4D data sets
3
Image Acquisition
Snap/movie acquisition
3 3 3
Time-lapse at specified interval
3 3
Automated multi-wavelength
3
Multichannel Acquisition
Z-Stack
3
Multi-dimensional (xyzt and wavelength)
3
Graphical Experiment Manager
3
Manual assisted panoramic imaging (manual MIA)
3
Manual Process
Multiposition acquisition and stage navigator Multiposition
Automated panoramic imaging (auto MIA, requires motorized stage) Multiposition
Instant EFI image (manual or motorized Z)
3
Manual Process
Simultaneous multi-color imaging (Image splitter needs)
Ratio/High End Device
Live deblurring
3
High Dynamic Range Imaging (HDRI)
3
Multi-well Plate Acquisition
Well Plate Navigator
Multiposition
Image Processing
Geometry/combine/filter processing
3 3
Fluorescence unmixing
3
Brightfield unmixing
3
Deblurring (No/Nearest Neighbor, Wiener Filter)
3
2D deconvolution (constrained iterative deconvolution)
3
3D deconvolution (constrained iterative deconvolution) CI Deconvolution
Image Analysis
Region and line measurements
3 3
Phase analysis
3
Object analysis and classification Count & Measure
Interactive measurement
3 3 3
*
Intensity plot over time/z
3
Colocalization
3
Object Counting (Manual)
3 3
Online Ratio and Kinetics Ratio
Ratio analysis (off-line)
3
Documentation and
Collaboration
Automatically compose Word reports
3
Database image and data management solution for microscopy Database Core Database Core
Save and load images/documents from Database Database Client Database Client Database Client
Remoting Remote Live Image Viewing NetCam
NetCam
* Three points angle, four points angle, arbitrary line, closed polygon, polyline and perpendicular line only.
and
Image data courtesy of:
Hiroo Ueno, Ph.D.
Department of Stem Cell Pathology, Kansai Medical University
(cover page)
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Summary of Contents

Page 1 - Imaging Software

Imaging SoftwarecellSensSeamless Workflow. Intuitive Operation.Compatible image formatsRead and write JPEG, JPEG2000, TIFF, BMP, AVI, PNG, VSI (Virtual

Page 2 - LEAVE MORE TIME FOR RESEARCH

12Olympus cellSens gives you a simpler way to work. Enjoy full control over the user interface, with functions that are where you want them, when you

Page 3 - Convenient Operation

34Convenient OperationREDUCE CLUTTER AND CONFUSION BY DISPLAYING ONLY THE TOOLS AND WINDOWS YOU NEEDArrange Windows as You LikeOrganize the tools and

Page 4 - EMPOWERED TO DO WHAT YOU WANT

56FunctionsEMPOWERED TO DO WHAT YOU WANTOur Solutions Our SolutionsGraphical Experiment Manager (GEM)This function allows experiments to be designed

Page 5 - Viewing and Processing

UnmixingWith the linear unmixing algorithm in cellSens Dimension, you can readily separate fluorochromes which overlap in their emission spectra—such

Page 6 - Measurement and Analysis

DatabaseThe Database Core Solution allows the creation of user-defined databases, with full access control, which can be shared across a network. The d

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